Effects of pH on benzimidazole fluorescence |
| |
Authors: | Kenneth S RogersCC Clayton |
| |
Institution: | Department of Biochemistry, Medical College of Virginia, Health Sciences Division of Virginia Commonwealth University, Richmond, Virginia 23219 USA |
| |
Abstract: | Four benzimidazoles (unsubstituted, 5-methyl, 2-ethyl, and 2-ethyl-5-methyl) have been characterized by fluorescence spectroscopy. At low pH (<6), activation at 270 nm caused fluorescence at 305 nm; at high pH (<8), activation at 270 nm caused fluorescence at 365 nm. The relative proportion of peak fluorescence at either 305 or 365 nm was correlated with the pKa values of the four benzimidazoles. It was concluded that the protonated specie of benzimidazole was fluorescent at 365 nm and the unprotonated specie was also fluorescent at 305 nm. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|