d-glucose uptake in human liver cell cultures |
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Authors: | F Lemonier M Feneant N Moatti M Gautier A Lemonnier |
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Institution: | 1. Unité de Recherche d'Hépatologie Infantile I.N.S.E.R.M. U 56 H?pital d'Enfants, 94270, Le Kremlin-Bicêtre, France 2. Laboratoire Central de Biochimie, Centre Hospitalier de Bicêtre, 94270, Le Kremlin-Bicêtre, France
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Abstract: | Summary The kinetic parameters ford-glucose uptake were studied in human liver cell cultures under strictly defined experimental conditions. Using a wide concentration
range (0.005 to 30 mmol/l), the kinetic data obtained suggested strongly thatd-glucose in human liver cell cultures can be transported by two separate systems. For the high-affinity system, the apparentK
m was 0.645±0.21 mmol/l and the Vmax, 12.49±3.74 nmol/mg protein per min. For the low-affinity system, the apparentK
m was 6.91±0.58 mmol/l and the Vmax, 79.90±5.27 nmol/mg protein per min. At a concentration of 2.1×10−7 mol/l, cytochalasin B preferentially inhibited the high-affinityd-glucose site or transport system. The time course ofd-glucose uptake, studied in two cell lines from patients with hereditary fructose intolerance, was significantly higher than
for the control lines.
This work was supported by Grant I.N.S.E.R.M. CRL 77-5-210-4. |
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Keywords: | D-glucose uptake human liver cells cultures cytochalasin B |
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