| 高通量测序及其在食物网解析中的应用进展 |
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| 引用本文： | 王雪芹,王光华,乔飞,高其康,Kong Luen HEONG,祝增荣,程家安.高通量测序及其在食物网解析中的应用进展[J].生态学报,2017,37(8):2530-2539. |
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| 作者姓名： | 王雪芹 王光华 乔飞 高其康 Kong Luen HEONG 祝增荣 程家安 |
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| 作者单位： | 浙江大学水稻生物学国家重点实验室, 杭州 310058;浙江大学昆虫科学研究所, 杭州 310058,浙江大学水稻生物学国家重点实验室, 杭州 310058;浙江大学昆虫科学研究所, 杭州 310058,浙江大学水稻生物学国家重点实验室, 杭州 310058;浙江大学昆虫科学研究所, 杭州 310058,浙江大学昆虫科学研究所, 杭州 310058;浙江大学农生环学部分析测试中心, 杭州 310058,浙江大学水稻生物学国家重点实验室, 杭州 310058;浙江大学昆虫科学研究所, 杭州 310058,浙江大学水稻生物学国家重点实验室, 杭州 310058;浙江大学昆虫科学研究所, 杭州 310058,浙江大学水稻生物学国家重点实验室, 杭州 310058;浙江大学昆虫科学研究所, 杭州 310058 |
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| 基金项目： | 国家科技支撑计划项目（2012BAD19B01）;浙江省自然科学基金项目（LY16C140002）;科技部国家"973"基础重点研究发展规划项目（2010CB126200） |
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| 摘 要： | 高通量测序是DNA测序技术发展中的重大突破,它的出现为现代生物科学研究提供了前所未有的机遇,例如基于猎物和寄主植物DNA分子解析生态系统的食物网研究已逐渐成为捕食性动物与植食性动物食物网研究的新型模式。在简要总结Roche 454、Illumina和Ion Torrent为代表的第二代测序技术的原理及最新进展的基础上,综述了近年来利用高通量测序技术在捕食性和植食性动物食物网解析构建研究方面取得的最新进展及存在的问题,以期为探索捕食性和植食性动物的猎物/寄主范围、猎物/寄主转换、资源分配、生物防治、生物保护和生态恢复新方法提供思路和启发。
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| 关 键 词： | 第二代测序 捕食/植食性动物 营养关系 食物转换 宏条形码技术 |
| 收稿时间： | 2015/11/16 0:00:00 |
| 修稿时间： | 2016/8/8 0:00:00 |
Progress on high-throughput sequencing and its applications in food web analysis |
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| WANG Xueqin,WANG Guanghu,QIAO Fei,GAO Qikang,Kong Luen HEONG,ZHU Zengrong and CHENG Jiaan.Progress on high-throughput sequencing and its applications in food web analysis[J].Acta Ecologica Sinica,2017,37(8):2530-2539. |
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| Authors: | WANG Xueqin WANG Guanghu QIAO Fei GAO Qikang Kong Luen HEONG ZHU Zengrong and CHENG Jiaan |
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| Institution: | State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China;Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China,State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China;Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China,State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China;Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China,Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China;Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China,State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China;Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China,State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China;Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China and State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China;Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | High-throughput sequencing is a major breakthrough in the development of DNA sequencing technology and provides an unprecedented opportunity for modern biological scientific research, such as the DNA-based approach tracking the food chains among predators-prey or herbivores-host plants trophic interactions in ecosystems. This review illustrates and compares the principles of various types of technology, including the Roche 454, Illumina, and Ion Torrent technologies, and other recent progress. We also summarize studies that have used high-throughput sequencing technology to study interactions among generalist predators/herbivores and their prey/hosts. This review could provide new information and novel approaches to exploring molecular trophic interactions and improving our understanding of the prey/host spectrum, prey/host shift, biological control, resource allocation, conservation biology, and ecological restoration. |
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| Keywords: | next generation sequencing generalist predator generalist herbivore trophic interactions diet switching metabarcoding |
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