| 基于空间频域滤波高动态光学投影层析的斑马鱼三维成像研究 |
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| 引用本文： | 李秉尧,张艳婷,林秋萍,王磊,黎思娜,韩定安,钟俊平,王茗祎,曾亚光. 基于空间频域滤波高动态光学投影层析的斑马鱼三维成像研究[J]. 生物化学与生物物理进展, 2019, 46(11): 1110-1117 |
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| 作者姓名： | 李秉尧 张艳婷 林秋萍 王磊 黎思娜 韩定安 钟俊平 王茗祎 曾亚光 |
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| 作者单位： | 佛山科学技术学院物理与光电工程学院，佛山 528000,佛山科学技术学院物理与光电工程学院，佛山 528000,佛山科学技术学院物理与光电工程学院，佛山 528000,佛山科学技术学院物理与光电工程学院，佛山 528000,佛山科学技术学院物理与光电工程学院，佛山 528000,佛山科学技术学院物理与光电工程学院，佛山 528000,佛山科学技术学院物理与光电工程学院，佛山 528000,佛山科学技术学院物理与光电工程学院，佛山 528000,佛山科学技术学院物理与光电工程学院，佛山 528000 |
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| 基金项目： | 国家自然科学基金(61771139,61605026,81601534,61805038,61705036)，广东省自然科学基金(2017A030313386)，佛山科学技术学院高层次人才科研启动项目(gg040988)，佛山科学技术学院高层次人才及岭南学者科研启动项目（肿瘤血管的多参数可视化研究，糖尿病视网膜微血管功能成像研究），2019年研究生自由探索基金(2019ZYTS45)，广东省大学生“攀登计划”专项资金(pdjh2019b0508)和佛山科学技术学院大学生创新创业训练计划(XJ2019253,XJ2019243)资助项目. |
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| 摘 要： | 本文提出了一种基于空间频率滤波的多曝光融合的高动态投影层析三维成像方法，实现了活体斑马鱼（17 mm × 4 mm，最大厚度为2.33 mm,最小厚度为0.29 mm）的三维结构成像. 通过相机采用不同曝光时间记录系列吸收图像，将每张图像取变换到频域去除低频后，将各张滤波后叠加并逆傅里叶变换回空域，对变换后的图像进行归一化处理，最终获得高动态图像. 在每个投影角度获得这种高动态吸收投影图像，进行滤波反投影算法重建，获得高动态的整条斑马鱼三维结构信息. 实验成像结果表明，这种空间频率滤波多曝光融合的高动态光学投影层析三维成像研究，可以获得复杂结构更丰富的空间信息，对斑马鱼等模式生物早期胚胎生长发育进程进行监测和定量评估有一定的应用前景.
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| 关 键 词： | 光学投影层析成像 高动态范围 频域 三维结构成像 |
| 收稿时间： | 2019-04-16 |
| 修稿时间： | 2019-08-11 |
High Dynamic Optical Projection Tomography Based on Multi-exposure for Three-dimensional Imaging of Zebrafish |
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| LI Bing-Yao,ZHANG Yan-Ting,LIN Qiu-Ping,WANG Lei,LI Si-N,HAN Ding-An,ZHONG Jun-Ping,WANG Ming-Yi and ZENG Ya-Guang. High Dynamic Optical Projection Tomography Based on Multi-exposure for Three-dimensional Imaging of Zebrafish[J]. Progress In Biochemistry and Biophysics, 2019, 46(11): 1110-1117 |
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| Authors: | LI Bing-Yao ZHANG Yan-Ting LIN Qiu-Ping WANG Lei LI Si-N HAN Ding-An ZHONG Jun-Ping WANG Ming-Yi ZENG Ya-Guang |
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| Affiliation: | School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China,School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China |
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| Abstract: | In this paper, a multi-exposure fusion high-motion projection tomography three-dimensional imaging method based on spatial frequency filtering is proposed to realize the three-dimensional structure imaging of living zebrafish(17 mm×4 mm, maximum thickness 2.33 mm, minimum thickness 0.29 mm). Our method is to record the series of absorption images by using different exposure time of the camera, transform each image into the frequency domain to remove the low frequency, and then superimpose each filtered spatial frequency signal and inverse Fourier transform back into the air domain, and the transformed image is normalized to obtain a high dynamic image. The high dynamic absorption projection image is obtained at each projection angle, and then reconstructed by a filtered back projection algorithm to obtain highly dynamic three-dimensional structure information. The experimental imaging results show that the multi-exposure fusion high-visibility optical projection tomography three-dimensional imaging study of spatial frequency filtering can obtain more abundant spatial information of complex structures, and the relationship and process of early embryo growth and development of zebrafish monitoring and quantitative assessment have certain application prospects. |
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| Keywords: | optical projection tomography high dynamics frequency domain three-dimensional imaging |
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