| 环境DNA metabarcoding及其在生态学研究中的应用 |
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| 引用本文： | 陈炼,吴琳,刘燕,徐海根. 环境DNA metabarcoding及其在生态学研究中的应用[J]. 生态学报, 2016, 36(15): 4573-4582 |
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| 作者姓名： | 陈炼 吴琳 刘燕 徐海根 |
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| 作者单位： | 环境保护部南京环境科学研究所, 南京 210042;江苏第二师范学院生命科学与化学化工学院, 南京 210013,江苏第二师范学院生命科学与化学化工学院, 南京 210013,环境保护部南京环境科学研究所, 南京 210042,环境保护部南京环境科学研究所, 南京 210042 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项（2013）；江苏省环境科技创新团队项目资助 |
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| 摘 要： | 环境DNA metabarcoding(eDNA metabarcoding)是指利用环境样本(如土壤、水、粪便等)中分离的DNA进行高通量的多个物种(或高级分类单元)鉴定的方法。近年来,该方法引起了学者的广泛关注,逐渐应用于生物多样性研究、水生生物监测、珍稀濒危物种和外来入侵物种检测等生态学领域。介绍环境DNA metabarcoding的含义和研究方法;重点介绍环境DNA metabarcoding在物种监测、生物多样性研究和食性分析等生态学领域中的应用;总结环境DNA metabarcoding应用于生态学研究领域面临的挑战并对该方法的发展进行展望。
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| 关 键 词： | 环境DNA metabarcoding 生物多样性 物种监测 |
| 收稿时间： | 2015-01-15 |
| 修稿时间： | 2016-05-16 |
Application of environmental DNA metabarcoding in ecology |
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| CHEN Lian,WU Lin,LIU Yan and XU Haigen. Application of environmental DNA metabarcoding in ecology[J]. Acta Ecologica Sinica, 2016, 36(15): 4573-4582 |
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| Authors: | CHEN Lian WU Lin LIU Yan XU Haigen |
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| Affiliation: | Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China;College of Life Sciences, Chemistry and Chemical Engineering, Jiangsu Second Normal University, Nanjing 210013, China,College of Life Sciences, Chemistry and Chemical Engineering, Jiangsu Second Normal University, Nanjing 210013, China,Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China and Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China |
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| Abstract: | Environmental DNA metabarcoding is defined as high throughput DNA sequencing used for simultaneous molecular identification of multiple taxa in a complex environmental sample (i.e., soil, water, feces, etc.). In recent years, this method has aroused widespread concern among scientists and has been increasingly used in the field of ecology, such as biodiversity research, monitoring of aquatic, rare, or invasive species. This article describes the significance and the methods of environmental DNA metabarcoding, and highlights its application in the fields of species monitoring,detection of rare and invasive species, biodiversity, and diet analysis. Finally, this article summarizes the challenges in application of environmental DNA metabarcoding in ecological research and discusses the future prospects of its development. |
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| Keywords: | environmental DNA metabarcoding biodiversity species detection |
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