| SPE-HPLC法快速检测发酵液中紫杉醇的含量 |
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| 引用本文： | 李勇超,许桂芳,杨靖,孟丽. SPE-HPLC法快速检测发酵液中紫杉醇的含量[J]. 生物技术通报, 2009, 0(12) |
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| 作者姓名： | 李勇超 许桂芳 杨靖 孟丽 |
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| 作者单位： | 河南科技学院生命科技学院,新乡,453003 |
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| 基金项目： | 河南省重点科技攻关，校科研启动基金 |
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| 摘 要： | 针对红豆杉内生真菌发酵液中紫杉醇的含量测定进行探讨,以建立快速高效低耗的检测方法.采用C_(18)固相萃取柱对紫杉醇进行吸附,用不同浓度的甲醇-乙酸铵和甲醇分别作为洗脱剂对其进行洗脱,比较两者的洗脱效果,洗脱液用HPLC进行检测;色谱条件为:流动相甲醇(v):水(v):乙腈(v)=20: 45: 35,流速:0.70 ml/min,检测波长:227 nm.结果表明,浓度为80%的甲醇溶液洗脱效果较好,紫杉醇的回收率为87.6%.
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| 关 键 词： | 紫杉醇 固相萃取 高效液相色谱 分离纯化 |
Determination of Paclitaxel in Fermentation Broth by Solid-phase Extraction and RP-HPLC |
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| Li Yongchao,Xu Guifang,Yang Jing,Meng Li. Determination of Paclitaxel in Fermentation Broth by Solid-phase Extraction and RP-HPLC[J]. Biotechnology Bulletin, 2009, 0(12) |
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| Authors: | Li Yongchao Xu Guifang Yang Jing Meng Li |
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| Abstract: | A simple, rapid and sensitive method for the determination of paclitaxel in endophytic fungi fermentation broth using solid phase extraction and high performance liquid chromatography was established.Paclitaxel was extracted with CH_2Cl_2.After being purified by C_(18) solid phase extraction, the purified extract was analyzed directly by high performance liquid chromatography on a C_(18) column and UV detector at 227 nm, with mixtures of methanol: water: acetonitrile (20:45:35) as mobile phase.The results show that methanol with content of 80% as eluant can gain high recovery with 87.6%. |
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| Keywords: | Paclitaxel Solid phase extraction (SPE) High performance liquid chromatography (HPLC) Extract and Purification |
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