梳唇石斛试管开花诱导和离体保存

对珍稀濒危药用植物梳唇石斛（Dendrobidium strongylanthum Rchb. f.）离体开花诱导和保存进行试验、观察和分析，研究基本培养基和植物生长调节剂对诱导离体开花的影响以及培养条件对离体保存的影响。结果显示，试管丛芽培养在MS＋6-BA 2 mg/L＋NAA 0.2 mg/L的培养基上，培养20 d试管开花的诱导率高达96%以上。试管丛芽接种在花宝1号3 g/L＋NAA 1 mg/L＋活性炭0.5 g/L培养基上，在12 ℃和HR 60%条件下保存，继代周期可延长至2年。该物种快速繁殖方法和保存技术的成功，为濒危植物梳唇石斛持续利用提供了技术支持。

In vitro Flowering and Conservation of Dendrobidium strongylanthum Rchb.f.

The In vitro flowering and conservation of Dendrobidium strongylanthum were conducted,observed and analyzed.The impact of medium and plant hormones on in vitro flowering and the influence of environment(at 12 ℃,HR 60%) on in vitro conservation were also studied.The results showed the induction rate of cluster buds in MS+6-BA 2 mg/L+NAA 0.2 mg/L with 3% Sucrose for 70 d was over 96%.Moreover,the cluster buds could be inoculated in hyponex 1#(C:P:K=7:6:19) 3 g/L+NAA 1 mg/L+AC 0.5 g/L with 2% Sucrose at 12 ℃,HR 60% to make subculture cycle for two years.The success of rapid propagation and In vitro conservation provided technical support for the sustainable utilization of Dendrobidium strongylanthum.