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不同浓度6-BA及KT对马铃薯无菌苗诱导的影响
引用本文:余如刚,杜雪玲,宋运贤. 不同浓度6-BA及KT对马铃薯无菌苗诱导的影响[J]. 亚热带植物科学, 2012, 41(2): 19-22. DOI: 10.3969/j.issn.1009-7791.2012.02.005
作者姓名:余如刚  杜雪玲  宋运贤
作者单位:淮北师范大学生命科学学院,资源植物学安徽省重点实验室,安徽 淮北 235000
基金项目:安徽省高等学校省级自然科学研究项目(KJ2009B064Z);淮北师范大学校青年项目(700295)资助
摘    要:以‘高原7 号’马铃薯茎尖为外植体,用0.1%升汞和75%酒精消毒,经组织培养获得脱毒马铃薯茎段,在增殖培养中,研究不同浓度6-BA 及KT 对马铃薯茎段诱导增殖效果的影响。结果表明:用0.1%升汞对茎尖消毒8 min 效果最好,灭菌率达96.67%。细胞分裂素对马铃薯茎段增殖的促进作用为6-BA>KT,以1.5 mg/L 6-BA 诱导不定芽及其生长势效果最佳。

关 键 词:马铃薯  茎尖培养  茎段  细胞分裂素  
收稿时间:2012-01-19
修稿时间:2012-01-19

Effects of Different Cytokinins on Induction of Potato Sterile Plantlet
YU Ru-gang,DU Xue-ling,SONG Yun-xian. Effects of Different Cytokinins on Induction of Potato Sterile Plantlet[J]. Subtropical Plant Science, 2012, 41(2): 19-22. DOI: 10.3969/j.issn.1009-7791.2012.02.005
Authors:YU Ru-gang  DU Xue-ling  SONG Yun-xian
Affiliation:College of Life Science, Huaibei Normal University, Huaibei 235000, Anhui China
Abstract:Shoot tips of ‘Highland No.7’ potato are selected as explants and 0.1% Mercuric Chloride and 75% alcohol are employed to disinfect;then through tissue culture,virus-free potato stems are obtained.In the process of enrichment culture,the paper tries to analyze the effect that 6-BA and KT with different consistency have on proliferation-inducing result of potato stems.The result indicates that it is the most effective to use 0.1% Mercuric Chloride to disinfect the shoot tips for 8 minutes,by which the disinfection rate can be up to 96.67%.The better promoting effect of potato stem proliferation made by cytokinin can be achieved when 6-BA > KT;adventitious bud induction and growth potential are the best when the consistency of 6-BA reaches 1.5 mg/L.
Keywords:potato  shoot tip culture  stem  cytokinin
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