| 不同浓度6-BA及KT对马铃薯无菌苗诱导的影响 |
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| 引用本文： | 余如刚,杜雪玲,宋运贤.不同浓度6-BA及KT对马铃薯无菌苗诱导的影响[J].亚热带植物科学,2012,41(2):19-22. |
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| 作者姓名： | 余如刚 杜雪玲 宋运贤 |
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| 作者单位： | 淮北师范大学生命科学学院，资源植物学安徽省重点实验室，安徽 淮北 235000 |
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| 基金项目： | 安徽省高等学校省级自然科学研究项目(KJ2009B064Z);淮北师范大学校青年项目(700295)资助 |
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| 摘 要： | 以‘高原7 号’马铃薯茎尖为外植体，用0.1%升汞和75%酒精消毒，经组织培养获得脱毒马铃薯茎段，在增殖培养中，研究不同浓度6-BA 及KT 对马铃薯茎段诱导增殖效果的影响。结果表明：用0.1%升汞对茎尖消毒8 min 效果最好，灭菌率达96.67%。细胞分裂素对马铃薯茎段增殖的促进作用为6-BA>KT，以1.5 mg/L 6-BA 诱导不定芽及其生长势效果最佳。
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| 关 键 词： | 马铃薯 茎尖培养 茎段 细胞分裂素 |
| 收稿时间： | 2012-01-19 |
| 修稿时间： | 2012-01-19 |
Effects of Different Cytokinins on Induction of Potato Sterile Plantlet |
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| YU Ru-gang,DU Xue-ling,SONG Yun-xian.Effects of Different Cytokinins on Induction of Potato Sterile Plantlet[J].Subtropical Plant Science,2012,41(2):19-22. |
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| Authors: | YU Ru-gang DU Xue-ling SONG Yun-xian |
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| Institution: | College of Life Science, Huaibei Normal University, Huaibei 235000, Anhui China |
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| Abstract: | Shoot tips of ‘Highland No.7’ potato are selected as explants and 0.1% Mercuric Chloride and 75% alcohol are employed to disinfect;then through tissue culture,virus-free potato stems are obtained.In the process of enrichment culture,the paper tries to analyze the effect that 6-BA and KT with different consistency have on proliferation-inducing result of potato stems.The result indicates that it is the most effective to use 0.1% Mercuric Chloride to disinfect the shoot tips for 8 minutes,by which the disinfection rate can be up to 96.67%.The better promoting effect of potato stem proliferation made by cytokinin can be achieved when 6-BA > KT;adventitious bud induction and growth potential are the best when the consistency of 6-BA reaches 1.5 mg/L. |
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| Keywords: | potato shoot tip culture stem cytokinin |
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