Cloning and identification of the H-2D
p gene |
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| Authors: | Mary J Macchi Jerold G Woodward Elizabeth McLaughlin-Taylor Johanna Griffin Leroy Hood Dr Jeffrey A Frelinger |
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| Institution: | (1) Department of Microbiology, The University of Southern California School of Medicine, 90033 Los Angeles, California;(2) Division of Biology, The California Institute of Technology, 91125 Pasadena, California;(3) Department of Microbiology, University of Alabama in Birmingham, 35294 Birmingham, Alabama;(4) Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 804 FLOB 231H, 27514 Chapel Hill, NC;(5) Present address: Department of Medical Microbiology and Immunology, University of Kentucky Medical School, 40536-0084 Lexington, KY |
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| Abstract: | We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H 1 restriction fragment present in the  12a DNA clone to the D-TL region of H-2
p. Only a single 8.8 kb Barn H1 fragment can be assigned to K
p by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of 12a is presented. |
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