Identification and characterization of chlorpyrifos-methyl and 3,5,6-trichloro-2-pyridinol degrading Burkholderia sp. strain KR100 |
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Authors: | Jun-Ran Kim Young-Joon Ahn |
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Institution: | (1) Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, Republic of Korea |
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Abstract: | A chlorpyrifos-methyl (CM) degrading bacterium (designated strain KR100) was isolated from a Korean rice paddy soil and was
further tested for its sensitivity against eight commercial antibiotics. Based on morphological, biochemical, and molecular
characteristics, this bacterium showed greatest similarity to members of the order Burkholderiales and was shown to be most
closely related to members of the Burkholderia cepacia group. Strain KR100 hydrolyzed CM to 3,5,6-trichloro-2-pyridinol (TCP) and utilized TCP as the sole source of carbon for
its growth. The isolate was also able to degrade chlorpyrifos, dimethoate, fenitrothion, malathion, and monocrotophos at 300 μg/ml
but diazinon, dicrotophos, parathion, and parathion-methyl at 100 μg/ml. The ability to degrade CM was found to be encoded
on a single plasmid of ~50 kb, pKR1. Genes encoding resistance to amphotericin B, polymixin B sulfate, and tetracycline were
also located on the plasmid. This bacterium merits further study as a potential biological agent for the remediation of soil,
water, or crop contaminated with organophosphorus compounds because of its greater biodegradation activity and its broad specificity
against a range of organophosphorus insecticides. |
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Keywords: | Organophosphorus insecticide Chlorpyrifos-methyl 3 5 6-Trichloro-2-pyridinol Burkholderia cepacia Insecticide degrading bacteria Plasmid |
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