Production and purification of staphylococcal nuclease in <Emphasis Type="Italic">Lactococcus lactis</Emphasis> using a new expression-secretion system and a pH-regulated mini-reactor |
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| Authors: | Nicolas Trémillon Nicolas Issaly Julien Mozo Thomas Duvignau Hervé Ginisty Eric Devic Isabelle Poquet |
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| Institution: | 1.GTP-Technology,Immeuble Biostep,Labège Cedex,France;2.INRAUMR1319 Micalis (Microbiologie de l'Alimentation au service de la Santé),Domaine de Vilvert,Jouy-en-Josas cedex,France |
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| Abstract: | Background Staphylococcal (or micrococcal) nuclease or thermonuclease (SNase or Nuc) is a naturally-secreted nucleic acid degrading enzyme
that participates in Staphylococcus aureus spread in the infected host. Purified Nuc protein can be used as an exogenous reagent to clear cellular extracts and improve
protein purification. Here, a recombinant form of Nuc was produced and secreted in a Gram-positive host, Lactococcus lactis, and purified from the culture medium. |
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