| 生物油脂高产菌株筛选方法研究 |
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| 引用本文： | 宋安东,冯 冲,王风芹,谢 慧.生物油脂高产菌株筛选方法研究[J].微生物学通报,2009,36(3):0383-0388. |
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| 作者姓名： | 宋安东 冯 冲 王风芹 谢 慧 |
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| 作者单位： | 1. 河南农业大学生命科学学院,河南,郑州,450002
2. 河南农业大学生命科学学院,河南,郑州,450002;中州大学实验管理中心,河南,郑州,450044 |
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| 基金项目： | 国家自然科学基金(No. 70741032); 国家农业成果转化资金项目(No. 2006D00070597); 中国博士后科学基金资助项目(No. 20060400797) |
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| 摘 要： | 本文采用脂肪粒计数法、苏丹III菌泥染色法、碳饥饿检出法等对生物油脂高产菌株进行了筛选比较研究。通过试验表明, 苏丹III菌泥染色法简便快捷, 其结果与菌体的油脂含量有较好的相关性, 此法是筛选产脂菌株的较理想的初筛方法。碳饥饿检出法准确性较高, 但过于繁琐; 相比之下, 脂肪粒计数法虽简便, 但缺乏准确性, 使用时必须同时考虑脂肪粒的大小。通过筛选, 获得了一株生物油脂高产菌株IFFI01368, 其菌体中油脂含量为54.25%。
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| 关 键 词： | 生物油脂 筛选方法 脂肪粒 油脂含量 |
Research of Screening Method of the High Bio-oil Production Microorganisms |
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| SONG An-Dong,FENG Chong,WANG Feng-Qin and XIE Hui.Research of Screening Method of the High Bio-oil Production Microorganisms[J].Microbiology,2009,36(3):0383-0388. |
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| Authors: | SONG An-Dong FENG Chong WANG Feng-Qin and XIE Hui |
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| Institution: | College of Life Science, Henan Agricultural University, Zhengzhou, Henan 450002, China;(1. College of Life Science, Henan Agricultural University, Zhengzhou, Henan 450002, China) (2. Experimental Management Centre, Zhongzhou University, Zhengzhou, Henan 450044, China);College of Life Science, Henan Agricultural University, Zhengzhou, Henan 450002, China;College of Life Science, Henan Agricultural University, Zhengzhou, Henan 450002, China |
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| Abstract: | Fat Particles Counting(FPC), Yeast Mud Dying by Sudan III(YMDSIII), Culture of Lacking Carbon(CLC) were compared and approached in the essay. The results show that YMDSIII is simple and direct, its results are better interrelated with oil-producing quantity of yeast. This method is a relatively ideal screening method on choosing oil-producing microorganisms. CLC is relatively accurate, but it is too tedious. By comparison, FPC is simple and convenient, but it is short of accuracy. When it is used, it must consider the size of fat particles. By screening, cellular oil content reached 54.25% (W/W). |
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| Keywords: | Bio-oil Screening methed Fat particles Oil contents |
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