Differential invertase activity and root growth between Cu-tolerant and non-tolerant populations in Kummerowia stipulacea under Cu stress and nutrient deficiency |
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| Authors: | Zhi-Ting Xiong Ting Wang Kun Liu Zhi-Zhen Zhang Jin-Hua Gan Yu Huang Ming-Jing Li |
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| Institution: | 1. Department of Biology Education, Seoul National University, Seoul 08826, Republic of Korea;2. Center for Education Research, Seoul National University, Seoul 08826, Republic of Korea;3. Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea;1. Department of Chemistry and Chemical Engineering, Huanghuai University, Zhumadian 463000, China;2. Department of Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;1. Intensive Care Unit, Royal Melbourne Hospital, Parkville, VIC, Australia;2. Melbourne Health and University of Melbourne, Australia;1. Research Department of Chemistry, Aditanar College of Arts and Science, Tiruchendur 628 216, Tamil Nadu, India;2. Department of Chemistry, Kamaraj College, Thoothukudi 628 003, Tamil Nadu, India;1. Department of Biology Education, Seoul National University, Seoul 151-748, Republic of Korea;2. Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea;1. Institut FEMTO-ST, CNRS, Univ. Bourgogne Franche-Comté, 25030 Besançon cedex, France;2. Center of Excellence in Molecular Imaging, Chiang Mai University, 50200 Chiang Mai, Thailand;3. INSERM CIC 1431, Besançon University Hospital, 2 place Saint-Jacques, 25000 Besançon, France |
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| Abstract: | There has been no study on key enzymes in sucrose cleavage in metallophyte plants so far, which may be crucial for the plants’ root growth and heavy-metal tolerance maintenance. Here, we tested the hypothesis that the roots of copper tolerant plants should manifest a higher activity of acid invertases that are rate-limiting in sucrose catabolism than non-tolerant plants both for supporting growth and for their maintaining tolerance under Cu stress. Two populations of Kummerowia stipulacea, one from an ancient waste heap at a Cu mine, and the other from a non-contaminated site, were used in the experiments. The plants were grown in 1/2-fold (control) or 1/20-fold (nutrient deficiency) Hoagland’ solution, with (Cu stress) or without (control) 10 μmol/L Cu2+. Plants from the mine proved to be of Cu tolerance. Cu exposure had a stronger inhibition on root growth and thus resulting in a lower root/shoot ratio in the plants of non-mine population compared to the mine population. Cu exposure showed a stronger inhibition of acid invertase activity of Cu non-tolerant plants than Cu-tolerant plants, while neutral/alkaline invertase was insensitive to Cu. A positive correlation between the activity of acid invertases and the root growth and root/shoot ratio was observed. The results indicated an important role of acid invertases in governing root growth and root/shoot biomass allocation in the plants of mine population. The results also suggested that the higher activities in acid invertases of mine population plants might at least partly associate with the plants’ Cu tolerance, and their higher activities in acid invertases in turn played an role in maintenance of the Cu tolerance by supplying carbon and energy for tolerance mechanisms. In addition, the results showed evidence that neutral/alkaline invertase might play a role in compensating for the depression in sucrose catabolism due to Cu-induced inhibition in acid invertases. |
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