Salinity-induced physiological and proteomic changes in Anabaena doliolum |
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| Authors: | AK Srivastava P Bhargava R Thapar LC Rai |
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| Institution: | 1. Department of Botany, School of Life Sciences, Mizoram University, Aizawl 796009, India;2. Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India;1. Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071000, P.R.China;2. Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, Baoding 071000, P.R.China;3. Tangshan Normal University, Tangshan 063000, P.R.China;1. Department of Fish, Wildlife and Conservation Ecology, New Mexico State University, Las Cruces, NM 88003, USA;2. Chemical Analysis and Instrumentation Laboratory, New Mexico State University, Las Cruces, NM 88003, USA;1. Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, PR China;2. Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 1366 West Altheimer Drive, Fayetteville, AR 72704, USA;1. Biology Department, Universitat de les Illes Balears, 07122 Palma, Balearic Islands, Spain;2. IMEDEA (CSIC-UIB), 07190 Esporles, Balearic Islands, Spain;3. Plant Physiology Laboratory, Universitat Autonoma de Barcelona, 08193 Barcelona, Spain;4. Faculty of Agriculture and Forestry, University Nacional de La Plata, (FCAyF, UNLP), Phytopathology Center for Research on Plant Pathology (CIDEFI) and National Council of Scientific and Technical Research (CONICET), 1900 La Plata, Buenos Aires, Argentina |
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| Abstract: | This study is the first to offer information on salinity-induced inhibition of physiological variables, changes in proteome, and induction of glycolate metabolism in Anabaena doliolum. A significant reduction in O2-evolution, carbon fixation, chlorophyll and NADPH/NADH level and increase in intracellular Na+ and respiration were observed following 150 mM NaCl treatment for 1 and 24 h. Interestingly, ATP content registered significant decrease after 1 h and recovery after 24 h treatment of 150 mM NaCl. Two-dimensional gel electrophoresis and MALDI-TOF MS detected a set of six proteins showing significant reproducible alterations, and homology with iron superoxide dismutase, superoxide dismutase (imported), phycocyanin alpha chain, elongation factor-Tu (EF-Tu), ribulose 1,5-bisphosphate carboxylase/oxygenase and phosphoribulokinase of Nostoc PCC7120. Increased RuBisCO and decreased carbon fixation suggested operation of glycolate metabolism. This was confirmed by accumulation of free and phospho-glyceric acid, increase in glycolate oxidase activity, glycine, serine and ammonium contents. Since peroxide generated in this pathway cannot be scavenged due to sensitivity of catalase to NaCl the organism fails to acclimatize under salt stress. |
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