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Evidence for thiol-induced enhanced in situ translocation of 14C-sucrose from source to sink in Brassica juncea
Authors:Ashish Kumar Srivastava  Narayan Singh Nathawat  Nemmara Krishnan Ramaswamy  Maheshwar Prasad Sahu  Govind Singh  Jagdish Shivraman Nair  Radha Krishna Paladi  Stanliaus Fransis D'Souza
Affiliation:1. Nuclear Agriculture and Biotechnology Division (Fiply), Bhabha Atomic Research Centre, Mumbai 400085, India;2. Directorate of Research, Rajasthan Agriculture University, Bikaner, Rajasthan, India;1. Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, Republic of Korea;2. Department of Agronomy, Gyeongsang National University, Jinju 660-701, Republic of Korea;1. College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China;2. College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China;1. Center for Education and Research on Macromolecules (CERM), University of Liege, Chemistry Department, Sart-Tilman, Allée de la Chimie 3, Bat B6a, B-4000 Liege, Belgium;2. Unit of Food Science and Formulation, University of Liege, Gembloux Agro-Bio Tech Passage des Déportés 2, B-5030 Gembloux, Belgium;1. Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743 Jena, Germany;2. Jena Center for Soft Matter (JCSM), Friedrich Schiller University, Philosophenweg 7, 07743 Jena, Germany;3. Dutch Polymer Institute (DPI), P.O. Box 902, 5600 AX Eindhoven, The Netherlands;1. UMR AGAP, INRA-Montpellier SupAgro-CIRAD, 2 Place Pierre Viala, F-34060 Montpellier, France;2. UMR1083 SPO, INRA-Montpellier SupAgro-UM1, 2 Place Pierre Viala, F-34060 Montpellier, France;3. UMT Geno-Vigne, 2 Place Viala, F-34060 Montpellier, France;1. Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China;2. Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
Abstract:The translocation of 14C-sucrose to the different parts of the mustard (Brassica juncea) crop has been evaluated in the context of understanding the source to sink relationship in the thiol-induced enhanced crop yield. The foliar application of thiols like TU, TGA and DTT to the plant gave maximum sucrose phosphate synthase activity, which was found to have direct correlation with the movement of sucrose. The distribution pattern of 14C-sucrose follows the path from internode and node to pod via leaf. The translocation of 14C-sucrose was found to be a light dependent process. Among the nucleotides ATP and GTP, only ATP was able to promote the translocation and GTP was ineffective. In this unique in situ tracer experiment using 14C-sucrose, we could establish that thiols are able to enhance the translocation of sucrose from source to sink.
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