Calcium protection of PS2 complex of Phaseolus coccineus from cadmium toxicity: In vitro study |
| |
| Authors: | Maria Drążkiewicz Tadeusz Baszyński |
| |
| Affiliation: | 1. IFISC (Institute for Cross-Disciplinary Physics and Complex Systems), CSIC-UIB, Palma de Mallorca, 07122 Spain;2. Climate Change Research Centre, University of New South Wales, Sydney 2052, Australia;3. ARC Centre of Excellence for Climate System Science, University of New South Wales, Sydney 2052, Australia;4. Laboratoire d’Etude en Géophysique et Océanographie Spatiales, CNRS/UPS/IRD/CNES, 14 av. E. Belin, Toulouse 31400, France;1. School of Environment, Tsinghua University, Beijing, 100084, China;2. College of Grassland and Environmental Science, Xinjiang Agricultural University, Urumqi, Xinjiang, 830052, China;1. Instituto Oceanográfico da Universidade de São Paulo (IO-USP), Pça. do Oceanográfico, 191, Butantã, São Paulo, SP 05508 120, Brazil;2. Instituto de Estudos Costeiros da Universidade Federal do Pará (IECos-UFPA), Al. Leandro Ribeiro, Aldeia, Braganca, PA 68600 000, Brazil;3. Centro de Estudos do Mar da Universidade Federal do Paraná (CEM-UFPR), Av. Beira-mar, P.O. Box 61, Balneário Pontal do Sul, Pontal do Paraná, PR 83255 976, Brazil;4. Centro de Tecnologia e Geociências da Universidade Federal de Pernambuco (CTG-UFPE), Av. Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE 50670 901, Brazil;5. Faculdad de Ciencias de la Universidad de La Republica, Iguá 4225, Malvín Norte, 11400 Montevideo, Uruguay;1. UNESP — Univ. Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias-FCAV, Depto. de Biologia Aplicada à Agropecuária (DBAA), 14884-900 Jaboticabal, SP, Brazil;2. Unifeob — Centro Universitário da Fundação de Ensino Octávio Bastos, 13874-149, São João da Boa Vista, SP, Brazil;3. USP — Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz-ESALQ, Depto. de Genética, 13418-900 Piracicaba, SP, Brazil;1. School of Land and Food, University of Tasmania, Hobart, TAS, Australia;2. Department of Agronomy, University of Agriculture, Faisalabad, Pakistan;3. College of Plant Science and Technology, Huazhong Agricultural University, Hubei, China;4. National Maize Key Laboratory, Department of Crop Biotechnology, School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China;5. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, 143005, Punjab, India |
| |
| Abstract: | The action of 10 and 20 mM Ca against harmful Cd effect on PS2 complex isolated from leaves of Phaseolus coccineus L. cv. Pi?kny Ja? was studied. The changes in fast chlorophyll a fluorescence induction kinetics and protein composition of PS2 complex were the symptoms of Cd toxicity and Ca protection of PS2 complex. Calcium applied at 10 mM concentration prevented F0 reduction caused by the presence of 250–1000 μM Cd in the incubation mixture, but that of (the variable chlorophyll a fluorescence) Fv, Fm, Fv/F0, and Fv/Fm only at 250 μM Cd. Ca concentration doubling in the incubation mixture resulted in complete overcoming the toxicity of 250–1000 μM Cd to Fv and Fm. However, the protection of Fv/F0 and the photochemical efficiency of PS2 (Fv/Fm) from 1000 μM Cd was only partial even at 20 mM Ca. A protective effect of 10 mM Ca on D1, D2 and 17 kDa proteins was found in PS2 complex exposed to 250 μM Cd, and on 43 kDa protein in the complex treated with 500 μM Cd. However, 20 mM Ca counteracted the toxicity of 500 μM Cd to the 43, 47 and 17 kDa proteins, as well as the harmful effect of 1000 μM Cd on 47 and 17 kDa ones. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
