Regeneration of albino shoots in cultured anthers of intermediate wheatgrass (Agropyron intermedium (Host) Beauv.) |
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Authors: | Kangshen Yao Chen H Chen Charles R McMullen |
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Institution: | (1) Department of Biology and Microbiology, South Dakota State University, 57007 Brookings, SD, USA |
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Abstract: | Shoots were regenerated from Oahe intermediate wheatgrass anthers cultured on Tsay's, N6, Yu-pei and 85D12 basal media supplemented with kinetin and 2,4-D or NAA. Androgenesis mainly started with symmetrical divisions of pollen nuclei immediately followed by cytokinesis. Formation of tetranucleate pollen grains resulting from asymmetrical divisions of the pollen nuclei was also noted. Tsay's medium was more effective for callus induction, while N6, Tsay's and Yu-pei differentiation media were equally effective for shoot regeneration in the calluses. All regenerants were albino.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
naphthaleneacetic acid |
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Keywords: | androgenesis sporophytic differentiation pathway |
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