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Energetics of glucose uptake in a Salmonella typhimurium mutant containing uncoupled enzyme IIGlc
Authors:G. J. G. Ruijter  P. W. Postma  K. van Dam
Affiliation:(1) E.C. Slater Institute for Biochemical Research, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands
Abstract:Uncoupled enzyme IIGlc of the phosphoenolpyruvate (PEP): glucose phosphotransferase system (PTS) in Salmonella typhimurium is able to catalyze glucose transport in the absence of PEP-dependent phosphorylation. We have studied the energetics of glucose uptake catalyzed by this uncoupled enzyme IIGlc. The molar growth yields on glucose of two strains cultured anaerobically in glucose-limited chemostat-and batch cultures were compared. Strain PP 799 transported and phosphorylated glucose via an intact PTS, while strain PP 952 took up glucose exclusively via uncoupled enzyme IIGlc, followed by ATP-dependent phosphorylation by glucokinase. Thus the strains were isogenic except for the mode of uptake and phosphorylation of the growth substrate. PP 799 and PP 952 exhibited similar YGlc values. Assuming equal YATP values for both strains this result indicated that there were no energetic demands for glucose uptake via uncoupled enzyme IIGlc.Abbreviations PTS phosphoenolpyruvate: carbohydrate phosphotransferase system - HPr histidine-containing phosphocarrier protein - GalP galactose permease
Keywords:Energetics (of glucose uptake)  Growth yield  Salmonella typhimurium  Uncoupled enzyme IIGlc  Glucose phosphotransferase system  Glucose transport
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