| Regulation Culture on Cytological，Biochemical and Physiological Characteristics of Somatic Carrot Em |
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| 引用本文： | 黄美娟,黄绍兴,朱溦.Regulation Culture on Cytological，Biochemical and Physiological Characteristics of Somatic Carrot Em[J].基因组蛋白质组与生物信息学报,1994(1). |
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| 作者姓名： | 黄美娟 黄绍兴 朱溦 |
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| 作者单位： | Departnient of Biology Peking University，Beijing 100871 |
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| 摘 要： | ＲｅｇｕｌａｔｉｏｎＣｕｌｔｕｒｅｏｎＣｙｔｏｌｏｇｉｃａｌ，ＢｉｏｃｈｅｍｉｃａｌａｎｄＰｈｙｓｉｏｌｏｇｉｃａｌＣｈａｒａｃｔｅｒｉｓｔｉｃｓｏｆＳｏｍａｔｉｃＣａｒｒｏｔＥｍｂｒｙｏｓＨＵＡＮＧＭｅｉ－ｊｕａｎ；（黄美娟），ＨＵＡＮＧＳｈａｏ－...
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Regulation Culture on Cytological, Biochemical and Physiological Characteristics of Somatic Carrot Embryos |
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| HUANG Mei-juan, HUANG Shao-xing, ZHU Cheng,.Regulation Culture on Cytological, Biochemical and Physiological Characteristics of Somatic Carrot Embryos[J].Genomics Proteomics & Bioinformatics,1994(1). |
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| Authors: | HUANG Mei-juan HUANG Shao-xing ZHU Cheng |
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| Abstract: | Cytological,biochemical and physiological charcteristics of quiescent,high-vigor carrot somatic embryos obtained with the use of regulation culture were studied. As shown in cytological observation, quiescent somatic embryos obtained by regulation culturing for 70 days displayed small compacted cells with thick wall, dense cytoplasm and large amount of starch granules. Biochemical tests revealed a starch content and a soluble sugar content 1.9 and 1.7 times respectively as much as those of the control embryos(normal culture) while physiological study demonstrated that the respiration was maintained stally at a very low level,only 1/4 that of the control at the end of the 70-day period. These results provide further evidences that regution culture so after the morphology, biochemistcy, physiologr and metabolism of somatic embryos that they became quiescent,highly synchronized, and had their roots ungrown, a sate facilitating safe storage. |
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| Keywords: | Carrot Somatic embryo Regulation culture Artificial seed |
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