Elimination ofMycoplasma hyorhinis infections from four cell lines |
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Authors: | Theodore Gurney Jr Margaret J Woolf Leslie J Abplanalp Nichole H McKittrick Jeanne N Dietz Barry C Cole |
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Institution: | (1) Department of Biology, University of Utah, 84112 Salt Lake City, Utah;(2) Division of Arthritis, University of Utah College of Medicine, 84132 Salt Lake City, Utah |
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Abstract: | Summary Four monolayer mammalian cell lines were cured ofMycoplasma hyorhinis infections by cloning in microtiter dishes in the presence of tetracycline and kanamycin. During cloning, cultures were refed
with fresh antibiotic containing medium every 2 or 3 d for 14 d and were then cultured without effective antibiotics for at
least 21 d. From the four lines we recovered 29 clones, none of which were infected after treatment as judged by the lack
of extranuclear fluorescence after staining with the fluorochrome Hoechst 33258, and by normal autoradiographic labeling of
the cells by tritiated nucleosides. One clone from each line was tested further by attempted culture of mycoplasmas and was
also judged to be uninfected. Infection has not reappeared in any of the clones after extensive culture in the absence of
the effective antibiotics.
This investigation was supported by Public Health Service Research Grant GM26137 from the National Institutes of General Medical
Sciences, National Institutes of Health, Bethesda, MD. |
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Keywords: | mycoplasma M hyorhinis infection curing cloning antibiotics |
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