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Effect of Aspergillus oryzae CBS 819.72 α-amylase on rheological dough properties and bread quality
Authors:Mouna Sahnoun  Belgacem Naili  Fatma Elgharbi  Radhouane Kammoun  Karima Gabsi  Samir Bejar
Institution:1. Laboratoire de Microorganisme et de Biomolécules, Centre de Biotechnologie de Sfax (LMB), Université de Sfax, route de Sidi Mansour Km 6, B.P 1177, Sfax, 3018, Tunisia
2. Laboratoire de recherche et de développement, Générale industrielle alimentaire Slama (GIAS), Bouargoub, 8040, Tunisia
3. Laboratoire d’analyse sensorielle et R&D, EuroSensoLab, Ariana, 2080, Tunisia
Abstract:An optimum Aspergillus oryzae CBS 819.72 α-amylase production in a laboratory-scale fermentor using a wheat grinding by-product as a sole carbon source (340 U/mL) was obtained after 48 h of batch fermentation under an agitation rate of 900 rpm and a pH maintained constant for 24 h. The application of this α-amylase preparation at an adequate concentration showed positive effects on dough properties and bread quality. Extensographic analysis revealed that while this addition induced a significant increase in maximal dough resistance to extension and area below the curve (energy), it brought a substantial decrease in extensibility. Farinographic results revealed small decreases in terms of time development, water absorption, and dough stability. Bread volume was also observed to undergo a significant increase.
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