Determination of amlodipine in human plasma by high-performance liquid chromatography with fluorescence detection |
| |
| Authors: | Sevgi Tatar Sedef Atmaca |
| |
| Affiliation: | 1. Department of Hospital Pharmacy, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands;2. Royal Dutch Pharmacists Association (KNMP), PO Box 30460, 2500 GL, Den Haag, The Netherlands;3. A15 Pharmacy, Buys Ballotstraat 2, 4207 HT Gorinchem, The Netherlands;1. Department of Psychology, Texas A&M University, College Station, TX, United States;2. Texas A&M Institute for Neuroscience, Texas A&M University, College Station, TX, United States;3. Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, United States;4. Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, United States |
| |
| Abstract: | A sensitive and specific HPLC method has been developed for the assay of amlodipine in human plasma. The assay involves derivatization with 4-chloro-7-nitrobenzofurazan (NBD-Cl), solid-phase extraction on a silica column and isocratic reversed-phase chromatography with fluorescence detection. Nortriptyline hydrochloride was used as an internal standard. The assay was linear over the concentration range of 0.25–18.00 ng/ml. Both of the within-day and day-to-day reproducibility and accuracy were less than 11.80% and 12.00%, respectively. The plasma profile following a single administration of 10 mg amlodipine to a healthy volunteer was presented. |
| |
| Keywords: | Amlodipine 4-chloro-7-nitrobenzofurazan |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
