Analysis of common epitopes on gag proteins of HIV-1, HIV-2 and SIV[AGM] using monoclonal antibodies against HIV-1. |
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| Authors: | M Asano S Morikawa M Tatsumi H Shimizu H Ushijima T Kitamura |
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| Institution: | Division of AIDS Virus, AIDS Research Center, National Institute of Health, Tokyo, Japan. |
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| Abstract: | Five monoclonal antibodies (MoAbs) to gag proteins of HIV-1 were prepared in mice. Western blot analyses showed that three clones recognized p24 and the other two p17. Among the three MoAbs recognizing p24, all recognized two of three strains of HIV-2. The spectra of reactions to SIVAGM] of these MoAbs against p24 were different from one to another; K3-24 recognized all four strains of SIVAGM], L6-24 three of them, and K5-24 none of them. Of the two MoAbs recognizing p17, K7-17 recognized two of the three strains of HIV-2 but not any SIVAGM] strain, and the other clone, L14-17 recognized none of analogous proteins of HIV-2 nor of SIVAGM]. These results demonstrate that the gag proteins of HIV-2 and SIVAGM] share some common epitopes with those of HIV-1 which are heterogenic in some degree among the different isolates. |
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