Cell-free protein synthesis for proteomics. |
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Authors: | Alison M Jackson Joe Boutell Neil Cooley Mingyue He |
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Affiliation: | Discerna Ltd., Babraham Research Campus, Babraham, Cambridge CB2 4AT, UK. |
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Abstract: | The use of cell-free expression systems as an alternative to cell-based methods for protein production is greatly facilitating studies of protein functions. Recent improvements to cell-free systems, and the development of cell-free protein display and microarray technologies, have led to cell-free protein synthesis becoming a powerful tool for large-scale analysis of proteins. This paper reviews the most commonly used cell-free systems and their applications in proteomics. |
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