Comparative AFLP mapping in two hexaploid oat populations |
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| Authors: | S Groh A Zacharias S F Kianian G A Penner J Chong H W Rines R L Phillips |
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| Institution: | (1) Department of Agronomy and Plant Genetics and Plant Molecular Genetics Institute, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108, USA e-mail: phill005@umn.edu Fax: +1 612-625-1268, US;(2) Cereal Research Centre, Agriculture and Agri-Food Canada, Winnipeg, MB R3 T 2M9, Canada, CA;(3) Department of Plant Sciences, North Dakota State University, Fargo, ND 58105, USA, US;(4) Plant Science Research Unit, USDA-ARS and Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA, US |
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| Abstract: | Amplified fragment length polymorphisms (AFLPs) can be used to quickly develop linkage maps in plant species and are especially
useful for crops with large genomes like oat (Avena sativa L., 2n=6x=42). High reproducibility and consistency are crucial if AFLP linkage maps are employed for comparative mapping.
We mapped AFLP markers in combination with restriction fragment length polymorphism (RFLP) markers in two recombinant inbred
populations of hexaploid oat in two laboratories to test the consistency of AFLP markers in a polyploid crop. Eight primer
combinations produced 102 and 121 scoreable AFLP markers in the respective populations. In a population from the cross Kanota×Ogle,
AFLP markers were placed onto a RFLP reference map consisting of 32 linkage groups. Nineteen linkage groups from another population
from the cross Kanota×Marion were assigned to the reference map using AFLP and RFLP markers homologous to those used in the
Kanota× Ogle cross. Reproducibility of AFLP assays was high in both laboratories and between laboratories. The AFLP markers
were well-distributed across the genome in both populations. Many AFLP markers tended to extend the distance between adjacent
RFLP markers in linkage analysis. Of the 27 polymorphic AFLPs common in both populations, 20 mapped to homologous linkage
groups, 4 were unlinked in at least one population, and 3 mapped to different linkage groups in the two crosses. We believe
that 1 of the 3 markers that mapped to a different linkage group in the two populations mapped to homoeologous linkage groups.
The linkage map of hexaploid oat is not yet complete, and genomic rearrangements such as translocations exist among cultivars
and are likely to account for the remaining two non-syntenous mapping results. AFLPs provide not only a fast and powerful
tool for mapping but could be useful in characterizing genomic structural variations among germplasms in hexaploid oat.
Received: 17 December 1999 / Accepted: 28 July 2000 |
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| Keywords: | Avena sativa AFLP Comparative mapping Homology Homoeology RFLP |
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