Quantification of prostaglandins in human seminal fluid |
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Authors: | K. Svanborg M. Bygdeman P. Eneroth E. Bendvold |
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Affiliation: | Research and Development Laboratory, Department of Obstetrics and Gynecology, Karolinska Hospital, S-104 01 Stockholm, Sweden |
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Abstract: | A method is described for measurement of the prostaglandins present in human seminal plasma. The PGEs and the 19-hydroxy-PGEs are converted to the respective PGB-compounds. They are determined with UV-absorbance after purification with two chromatography steps. The PGFs and the 19-hydroxy-PGFs are determines by gas chromatography, which also allows measurement of the 8β-isomers. Recovery of added PG was in the average 99.2% (range 89.9–107.7). Mean variation between duplicate analyses was 5.6% (range 0.9–9.1). The method has been simplified to allow at least limited clinical use. |
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