Alcoholic fermentation by wild-type <Emphasis Type="Italic">Hansenula polymorpha</Emphasis> and <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> versus recombinant strains with an elevated level of intracellular glutathione |
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Authors: | Dorota Grabek-Lejko Olena O Kurylenko Vladimir A Sibirny Vira M Ubiyvovk Michel Penninckx Andriy A Sibirny |
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Institution: | (1) University of Rzeszow, Cwiklinskiej 2, 35-601 Rzeszow, Poland;(2) Institute of Cell Biology NAS of Ukraine, Drahomanov Street, 14/16, Lviv, 79005, Ukraine;(3) Laboratory of Microbial Physiology and Ecology, Universite Libre de Bruxelles, 642 rue Engeland, 1180 Brussels, Belgium; |
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Abstract: | The ability of baker’s yeast Saccharomyces cerevisiae and of the thermotolerant methylotrophic yeast Hansenula polymorpha to produce ethanol during alcoholic fermentation of glucose was compared between wild-type strains and recombinant strains
possessing an elevated level of intracellular glutathione (GSH) due to overexpression of the first gene of GSH biosynthesis,
gamma-glutamylcysteine synthetase, or of the central regulatory gene of sulfur metabolism, MET4. The analyzed strains of H. polymorpha with an elevated pool of intracellular GSH were found to accumulate almost twice as much ethanol as the wild-type strain
during glucose fermentation, in contrast to GSH1-overexpressing S. cerevisiae strains, which also possessed an elevated pool of GSH. The ethanol tolerance of the GSH-overproducing strains was also determined.
For this, the wild-type strain and transformants with an elevated GSH pool were compared for their viability upon exposure
to exogenous ethanol. Unexpectedly, both S. cerevisiae and H. polymorpha transformants with a high GSH pool proved more sensitive to exogenous ethanol than the corresponding wild-type strains. |
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