Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1) interacts with secretory clusterin |
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| Authors: | Yan Zhou Leike Li Longbo Hu Tao Peng |
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| Institution: | (1) State Key Laboratory of Respiratory Diseases, Laboratory of Viral Immunology, Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, 510530, People’s Republic of China;(2) University of Science and Technology of China, Hefei, 230026, People’s Republic of China;(3) Present address: Guangzhou Institute of Biomedicine and Health, #190 Kai Yuan Avenue, Guangzhou Science Park, 510530, People’s Republic of China; |
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| Abstract: | Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1), a type-II Golgi transmembrane protein of unknown function, is up-regulated in
many cancers. Its Golgi luminal domain is potentially the major functional domain. The goal of this study is to identify the
proteins interacting with GOLPH2. Using secretory GOLPH2 (sGOLPH2, amino acid residues 55–401) as bait, secretory clusterin
(sCLU) was identified as one interacting candidate by yeast two-hybrid screening, and the coiled-coil domain of GOLPH2 was
found to be sufficient for interaction with sCLU. The interaction between GOLPH2 and sCLU was confirmed intracellularly and
extracellularly. The intracellular co-localization of GOLPH2 and sCLU in Golgi was also shown. These results can help in understanding
the biological and pathological significance of GOLPH2. |
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