Targeting xa13, a recessive gene for bacterial blight resistance in rice |
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Authors: | Zhaohui Chu Binying Fu Hong Yang Caiguo Xu Zhikang Li A. Sanchez Y. J. Park J. L. Bennetzen Qifa Zhang Shiping Wang |
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Affiliation: | (1) National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070 Wuhan, China;(2) Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, P. O. Box 933, 1099 Manila, The Philippines;(3) Department of Genetics, University of Georgia, Athens, GA 30602, USA;(4) Present address: Chinese Academy of Agricultural Sciences, Institute of Crop Sciences, 100081 Beijing, China |
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Abstract: | Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice worldwide. Thirty bacterial blight resistance (R) genes (21 dominant genes and 9 recessive genes) in rice have been identified. They are the main sources for the genetic improvement of rice for resistance to Xoo. However, little is known about the recessive R genes. To clone and characterize the recessive R genes, we fine-mapped xa13, a fully recessive gene for Xoo resistance, to a DNA fragment of 14.8 kb using the map-based cloning strategy and a series of sequence-based molecular markers. Sequence analysis of this fragment indicated that this region contains only two apparently intact candidate genes (an extensin-like gene and a homologue of nodulin MtN3) and the 5′ end of a predicted hypothetical gene. These results will greatly facilitate the isolation and characterization of xa13. Four PCR-based markers, E6a, SR6, ST9 and SR11 that were tightly linked to the xa13 locus, were also developed. These markers will be useful tools for the marker-assisted selection of xa13 in breeding programs. |
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