Effect of medium acidity on growth and rooting of different plant species growing in vitro |
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| Authors: | C Leifert Samantha Pryce P J Lumsden W M Waites |
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| Institution: | (1) Department of Cell and Structural Biology, Manchester University, Stopford Building, M13 9PT Manchester, UK;(2) Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, Sutton Bonington, LE12 5RD Loughborough, UK;(3) School of Applied Biology, Lancashire Polytechnic, PR1 2TQ Preston, UK;(4) Neo Plants Ltd., PR4 1HU Freckleton, Lancashire, UK |
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| Abstract: | Micropropagated Choisya, Daphne, Delphinium, Hemerocallis, Hosta, Iris and Photinia were found to adjust the pH of Murashige and Skoog's plant tissue culture medium (initial pH 5.6 or 3.5) to different values depending on the species. When plant growth and rooting rates were determined after plants had been grown on media initially adjusted or buffered to values between 2.6 and 5.7 the different plant species were also found to have distinct pH requirements for optimal growth and/or rooting rates.Abbreviations MS
Murashige & Skoog's (1962) medium
- MS19
MS with additionally 10 g l–1 sucrose
- 80 mg l–1
adenine sulphate and 130.9 mg l–1 NaH2PO4
- BA
6-benzyladenine
- NAA
1-naphthyl-acetic acid
- IBA
3-indole-butyric acid
- IAA
3-indole-acetic acid
- 2iP
N6(2-isopentyl) adenine |
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| Keywords: | medium pH micropropagation plant tissue culture |
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