Development of [3H]muscimol binding to subcellular particles of a culture of mouse brain |
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| Authors: | L L Sarliève F V DeFeudis L Ossola V Varga P Mandel |
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| Institution: | (1) Centre de Neurochimie du CNRS, Faculté de Médecine, 67085 Strasbourg Cedex, France;(2) Institute de Chimie Biologique, Faculté de Médecine, 67085 Strasbourg Cedex, France;(3) Present address: 1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary |
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| Abstract: |  Specific binding of 3H]muscimol (11.5 nM), related to GABA receptors, occurred in subcellular particles prepared from 4-, 8-, and 12-day cultures of embryonic (14-to 15-day-old) mouse cerebrum, but not to particles prepared from 20-to 45-day cultures. Phase-contrast microscopy revealed that the presence of neuronal elements (neuroblasts, neurites) paralleled the binding of 3H]muscimol. Further evidence is thus provided for using high-affinity 3H]muscimol binding as a neuronal marker.Supported by a UNESCO/IBRO fellowship |
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