Production of recombinant hIL-4delta2-a native isoform of human interleukin-4 in Escherichia coli cells |
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Authors: | Ptitsyn L R Smirnov S V Al'tman I B Samsonova N N Khodiakova A V Vasilenko R N |
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Affiliation: | Russian Federation State Research Center Genetika, Moscow, Russia. mashko@vnigen.msk.su |
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Abstract: | Expression plasmids containing the synthetic gene hil-4 delta 2 was constructed to produce human interleukin-4 in Escherichia coli cells. Strains TG1 (pBTIL-4 delta 2) and BL21 (DE3) (pETIL-4 delta 2) produced the recombinant protein as inclusion bodies, and its production level was up to 30% of the total cell protein. The renatured hIL-4 delta 2 inhibited IL-4-stimulated T cell proliferation, and this effect was enhanced by cyclosporin A. |
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