短尾猴与红面猴细胞色素b基因变异特点及系统发育分析

测定了2个短尾猴和1个红面猴个体的线粒体细胞色素b基因全序列,与猕猴、食蟹猴和叟猴的序列合并比较,分析了核苷酸序列差异和碱基替换特点,并以阿拉伯狒狒为外群,构建系统发生树。结果表明,短尾猴基因序列中A、T、G、C的含量分别为29.0%、25.5%、11.8%、33.6%,红面猴中A、T、G、C的含量分别为29.4%、24.3%、11.4%、34.9%,碱基组成具有哺乳动物的共同特点,短尾猴与红面猴序列间的同源性为90.1%。红面猴与食蟹猴之间的同义替换和异义替换值(Ks、Ka)以及Kimura双参数距离都要小于红面猴与短尾猴之间的差异值,在构建的系统发生树中,叟猴最早分化出来,红面猴并未与短尾猴聚为一支,而是与猕猴、食蟹猴聚在了一起。

Divergence and phylogeny of mitochondrial cytochrome b gene from Tibetan macaque and stump-tailed macaque

The complete mitochondrial cytochrome b genes were sequenced from two individuals of Tibetan macaque (Macaca thibetana) and one individual of stump-tailed macaque(Macaca arctoides).Through comparison with three other macaque species, the base composition and sequence divergence among these sequences were analyzed.The phylogenetic tree was constructed with Papio hamadryas as the outgroup.The contents of A、T、G、C were 29.0%、25.5%、11.8%、33.6% and 29.4%、24.3%、11.4%、34.9% in the cyt b gene sequences of the Tibetan macaque and stump-tailed macaque,respectively.The base composition of the Tibetan macaque and stump-tailed macaque showed the same character as that of other mammals.There was 90.1% homology with 113 variant sites between the two sequences.Results showed apparent divergence between Tibetan macaque and stump-tailed macaque.The phylogenetic tree supported the topology that Macaca sylvanu diverged firstly from other macaque species,and Macaca arctoides gathered together with M.mulatta and M.fascicularis rather than with M.thibetana.