紫孢侧耳、糙皮侧耳及其融合菌株的同工酶分析

采用聚丙烯酰胺凝胶垂直板电泳方法对紫孢侧耳、糙皮侧耳及其融合菌株的酯酶和过氧化物酶同工酶进行了分析。经多次试验,得到了两亲株和融合株恒定的同工酶谱, 在酶谱中融合菌株与亲株有明显差异,并表现出典型的双亲株互补酶带特征,从而证实融合菌株为两亲株基因重组之产物。 Abstract: After many repetitions,the patterns of esterase isozyme and peroxidase isozyme of Pleurotus sapidus,Pleurotus ostreatus and their fusant strains were obtained.There were differences among the parent strains and theirfusant strains in theirisozyme bands.Parental isozyme bands appeared in patterns of fusant strains.Itdemonstrated that fusant were recombinants of P.sapidus and P.ostreatus.

Studies on Isozyme of Pleurotus sapidus,Pleurolus ostreatus and Their Fusant Strains