| 黄鳝二价体上随机引物原位DNA合成的探讨 |
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| 引用本文： | 李奎,刘应伯,余其兴,赵则春,余先觉LI Kui,LIU Ying-Bo,YU Qi-Xing,ZHAO Ze-Chun,YU Xian-Jue. 黄鳝二价体上随机引物原位DNA合成的探讨[J]. 遗传, 1995, 17(1): 1-3 |
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| 作者姓名： | 李奎 刘应伯 余其兴 赵则春 余先觉LI Kui LIU Ying-Bo YU Qi-Xing ZHAO Ze-Chun YU Xian-Jue |
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| 作者单位： | 武汉大学生命科学学院遗传学系,武汉 430072Department of Genetics,College of Biology,Wuhan University,Wuhan 430072 |
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| 基金项目： | 国家自然科学基金，博士点科研基金 |
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| 摘 要： | 采用随机引物,在黄鳝二价体上建立了氚标记引物原位DNA合成技术,并详细探索了该技术的实验条件。其结果显示黄鳝二价体上DNA延伸效果十分明显,银颗粒基本上是非随机性的相间排列,而且主要特征是基本稳定的,据此可以进行染色体识别。此外,还讨论了建立该技术的重要意义。Abstract:We have developed a technique of random-primed tritium labeled DNA extension on the pachytene bivalents of rice-field eels.The bivalents were not homogeneously labeled with this technique and the extension signals were very apparent and relatively stable,and these can be applied to identify the individual bivalent.The significance to develop this technique has also been discussed in detail.
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| 关 键 词： | 氚 黄鳝 Key words Primed in situ DNA synthesis 二价体 引物原位DNA合成 遗传分化 布依族和水族 汉族 苗族 mtDNA多态性 |
Random-primed in situ Tritium Labeled DNA Synthesis on Pachytene Bivalents of Rice-field Eels (Manopterus albus Zuiew) |
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| $Li Kui, Liu Yingbo, Yu Qixing, Zhao Zechun, Yu Xianjue. Random-primed in situ Tritium Labeled DNA Synthesis on Pachytene Bivalents of Rice-field Eels (Manopterus albus Zuiew)[J]. Hereditas, 1995, 17(1): 1-3 |
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| Authors: | $Li Kui Liu Yingbo Yu Qixing Zhao Zechun Yu Xianjue |
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| Abstract: | We havc developed a techniquc of random-primcd tritium labeled DNA extension on thepachytene bivalents of rice-field ecls. The bivalents were not homogeneously labcled with this techniqueand the cxtension signa1s werc very apparent and relativcly stable, and thcse can be applied to identify theindividual bivalent. The significance to devclop this tcchniquc has also bcen discussed in detail. |
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| Keywords: | Primed in situ DNA synthesis Bivalents Tritium Manopterus albus |
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