| PCR方法用于奶牛早期胚胎的性别鉴定 |
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| 引用本文： | 杨建民,朱苏玲,武立红,蒋耀青,谭丽玲,宫云浩,孙淑芳,廖和模,严忠全YANG Jian-Min,ZHU Su-Ling,WU Li-Hong,JIANG Yao-Qing,TAN Li-Ling,GONG Yun-Hao,SUN Su-Fang,LIAO He-Mo,YAN Zhong-Quan.PCR方法用于奶牛早期胚胎的性别鉴定[J].遗传,1995,17(2):14-16. |
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| 作者姓名： | 杨建民 朱苏玲 武立红 蒋耀青 谭丽玲 宫云浩 孙淑芳 廖和模 严忠全YANG Jian-Min ZHU Su-Ling WU Li-Hong JIANG Yao-Qing TAN Li-Ling GONG Yun-Hao SUN Su-Fang LIAO He-Mo YAN Zhong-Quan |
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| 作者单位： | 1.中国学院遗传研究所, 北京100101
2.华南师范大学生物系，广州 510000
3.农业部动物检疫所，青岛 266000
4.四川省成都凤凰山乳牛场，成都 610081
1.Institute of Genetics,Academia Sinica,Beijing 100101
2.Department of Biology,Huanan Normal University,Guangzhou 510000
3.Institute of Animal Quarantine,The Ministry of Agriculture,Qingdao 266000
4.Fenghuangshan Dairy Farm,Chengdu,Sichuan 610081 |
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| 摘 要： | 根据牛Y染色体上的特异DNA序列合成一对引物, 通过PCR反应对奶牛早期胚胎进行性别鉴定。预测性别与移植胚胎产犊的实际性别相符率为80%。
Abstract:We have obtained the specific DNA segment from the bovine Y-chromosome and used it to design a pair of primer.The sex of embryos at the preimplantation stage have determined by using the polymerase chain reaction.10 months after uterine transfer showed that the rate of accuracy is 80%.
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| 关 键 词： | PCR 奶牛胚胎 PCR Y染色体 Key words 性别鉴定 自交可育性 再生植株 属间杂种 普通小麦 冰草 染色体配对 |
Sexing Bovine Embryos Using Polymerase Chain Redction |
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| YANG Jian-Min,ZHU Su-Ling,WU Li-Hong,JIANG Yao-Qing,TAN Li-Ling,GONG Yun-Hao,SUN Su-Fang,LIAO He-Mo,YAN Zhong-Quan.Sexing Bovine Embryos Using Polymerase Chain Redction[J].Hereditas,1995,17(2):14-16. |
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| Authors: | YANG Jian-Min ZHU Su-Ling WU Li-Hong JIANG Yao-Qing TAN Li-Ling GONG Yun-Hao SUN Su-Fang LIAO He-Mo YAN Zhong-Quan |
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| Abstract: | |
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| Keywords: | Chromosome pairing Agropyron cristatum(L )Gaertin Intergeneric hybrids Regenreated plants Self-fertility Triticum aestivumL |
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