Thermotropic ‘two-stage’ liquid crystalline crystalline lipid phase separation in microsomal membranes |
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Authors: | J Funk F Wunderlich W Kreutz |
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Institution: | Institut für Biophysik und Strahlenbiologie der Universität Freiburg im Breisgau, Albertstrasse 23, D-7800 Freiburg i. Br. F.R.G. |
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Abstract: | The effect of temperature on native microsomal membrane vesicles isolated from Tetrahymena is investigated by wide angle X-ray diffraction. A 4.2 Å reflection, typical for lipids in the crystalline state, can be recorded in the temperature range between 0°C and 35°C. Quantitative evaluation of this reflection reveals a broad thermotropic ‘two-stage’ liquid crystallinecrystalline lipid phase separation with a ‘breakpoint’ at approx. 18°C. This ‘breakpoint’ coincides with the emergence of lipid-protein segregations in endomembranes of intact Tetrahymena cells as previously visualized by freeze-etch electron microscopy. |
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Keywords: | Membrane vesicle Phase transition X-ray diffraction (T pyriformis) |
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