Genetic utility of natural history museum specimens: endangered fairy shrimp (Branchiopoda,Anostraca) |
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Authors: | Adam R Wall Daniel Campo Regina Wetzer |
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Institution: | 1.Natural History Museum of Los Angeles County, 900 Exposition Boulevard, Los Angeles, CA 90007 USA ;2.University of Southern California, Molecular and Computational Biology, Los Angeles, CA 90089 USA |
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Abstract: | We examined the potential utility of museum specimens as a source for genetic analysis of fairy shrimp. Because of loss of their vernal pool habitat, some fairy shrimp (including Branchinecta
sandiegonensis and Branchinecta
lynchi) are listed as threatened or endangered in Southern California by the United States Fish and Wildlife Service. Management of those species requires extensive population genetics studies and the resolution of important genetic complexity (e.g. possible hybridization between endangered and non-endangered species). Regulations mandating deposition of specimens of listed species have resulted in thousands of specimens accessioned into the Natural History Museum of Los Angeles County that have been preserved in a variety of solutions. We subsampled those specimens, as well as other Anostraca with known collection and preservation histories, to test their potential for genetic analysis by attempting DNA extraction and amplification for mt16SrDNA. Fixation and preservation in not denatured ethanol had a far greater sequencing success rate than other (and unknown) fixatives and preservatives. To maximize scientific value we recommend field preservation in 95% not denatured ethanol (or, if pure ethanol is unavailable, high-proof drinking spirits, e.g. Everclear™, or 151 proof white rum), followed by storage in 95% not denatured ethanol. |
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Keywords: | Museum specimens Anostraca" target="_blank">Anostraca Branchinectidae" target="_blank">Branchinectidae Branchinecta
sandiegonensis" target="_blank">Branchinecta
sandiegonensis Branchinecta
lynchi" target="_blank">Branchinecta
lynchi Branchinecta
lindahli" target="_blank">Branchinecta
lindahli endangered threatened vernal pool California |
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