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Genetic diversity of chloroquine-resistant Plasmodium vivax parasites from the western Brazilian Amazon |
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| Authors: | Omaira Vera Lizcano Sarah Stela Resende Yonne F Chehuan Marcus VG Lacerda Cristiana FA Brito Mariano G Zalis |
| Institution: | 1. Laboratório de Infectologia e Parasitologia Molecular, Serviço de Patologia Clínica, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil;2. Laboratório de Malária, Centro de Pesquisa René Rachou-Fiocruz, Belo Horizonte, MG, Brasil;3. Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Manaus, AM, Brasil |
| Abstract: | The molecular basis of Plasmodium vivax chloroquine (CQ) resistance is still unknown. Elucidating the molecular background of parasites that are sensitive or resistant to CQ will help to identify and monitor the spread of resistance. By genotyping a panel of molecular markers, we demonstrate a similar genetic variability between in vitro CQ-resistant and sensitive phenotypes of P. vivax parasites. However, our studies identified two loci (MS8 and MSP1-B10) that could be used to discriminate between both CQ-susceptible phenotypes among P. vivax isolates in vitro. These preliminary data suggest that microsatellites may be used to identify and to monitor the spread of P. vivax-resistance around the world. |
| Keywords: | Plasmodium vivax chloroquine resistance |