Proteomic Analysis of Kidney in Rats Chronically Exposed to Monosodium Glutamate |
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Authors: | Amod Sharma Chaisiri Wongkham Vitoon Prasongwattana Piyanard Boonnate Raynoo Thanan Sirirat Reungjui Ubon Cha’on |
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Affiliation: | 1. Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.; 2. Department of Internal Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.; Emory University, United States of America, |
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Abstract: | BackgroundChronic monosodium glutamate (MSG) intake causes kidney dysfunction and renal oxidative stress in the animal model. To gain insight into the renal changes induced by MSG, proteomic analysis of the kidneys was performed.MethodsSix week old male Wistar rats were given drinking water with or without MSG (2 mg/g body weight, n = 10 per group) for 9 months. Kidneys were removed, frozen, and stored at –75°C. After protein extraction, 2-D gel electrophoresis was performed and renal proteome profiles were examined with Colloidal Coomassie Brilliant Blue staining. Statistically significant protein spots (ANOVA, p<0.05) with 1.2-fold difference were excised and analyzed by LC-MS. Proteomic data were confirmed by immunohistochemistry and Western blot analyses.ResultsThe differential image analysis showed 157 changed spots, of which 71 spots were higher and 86 spots were lower in the MSG-treated group compared with those in the control group. Eight statistically significant and differentially expressed proteins were identified: glutathione S-transferase class-pi, heat shock cognate 71 kDa, phosphoserine phosphatase, phosphoglycerate kinase, cytosolic glycerol-3-phosphate dehydrogenase, 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, α-ketoglutarate dehydrogenase and succinyl-CoA ligase.ConclusionThe identified proteins are mainly related to oxidative stress and metabolism. They provide a valuable clue to explore the mechanism of renal handling and toxicity on chronic MSG intake. |
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