体内连续进化——从噬菌体到真核基因组的进化故事（英文）

进化是生物多样性产生和保留的自然进程。通过对编码蛋白质的基因进行有目的地设计和改造，获得性能更优异的蛋白质用于生产生活，是蛋白质工程的目的所在。为了在实验室中通过定向进化的蛋白质工程模拟自然进化的实现过程，研究人员通过在快速增殖的原核生物和简单的真核生物中引入靶向诱变元件，建立了各种体内连续进化系统。本综述介绍了体内连续进化平台的现状，重点关注噬菌体和酵母中人工进化技术的研究进展，并对其在生物技术领域中的成功应用进行了总结，最后简要展望了体内连续进化这一新兴领域的发展方向。

In vivo Continuous Evolution

Evolution is the natural history where biological diversity is generated and retained. To expedite the evolution process in the lab to achieve specific functional optimization by artificial selection, targeted mutagenesis boosts are introduced into fast-proliferating prokaryotes and simple eukaryotes. If tolerated, the functional diversity a fast-evolving system generated offers unique opportunities to obtain artificially engineered bio-molecules best suited for bioengineering applications. In this perspective, we discuss the current state of art in vivo continuous evolution platforms, focusing on advances made in both phage and yeast artificial evolution. Their successful applications in biotechnology are presented, followed by a brief outlook of near-future developments in this burgeoning field.