Genotypic and Phenotypic Differences between Nodal and Extranodal Diffuse Large B-Cell Lymphomas |
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Authors: | Salah A Al-Humood Aisha S Al-Qallaf Salem H AlShemmari Issam M Francis Thamradeen A Junaid Rajaa A Marouf Fahd Al-Mulla |
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Institution: | Department of Pathology, Faculty of Medicine, Kuwait University, Safat, Kuwait (SAA,ASA,IAF,TAJ,RAM,FA);Department of Medicine, Faculty of Medicine, Kuwait University, Safat, Kuwait (SHA) |
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Abstract: | Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous group of diseases that have diverse clinical, pathological, and biological features. Here, it is shown that primary nodal and extranodal DLBCLs differ genomically and phenotypically. Using conventional comparative genomic hybridization (CGH), the authors assessed the chromosomal aberrations in 18 nodal, 13 extranodal, and 5 mixed DLBCLs. The results demonstrate significantly distinct chromosomal aberrations exemplified by gains of chromosomal arms 1p, 7p, 12q24.21-12q24.31, and 22q and chromosome X and loss of chromosome 4, 6q, and 18q22.3-23 in extranodal compared with nodal DLBCLs. Nodal DLBCLs showed an increased tendency for 18q amplification and BCL2 protein overexpression compared with extranodal and mixed tumors. Using a panel of five antibodies against GCET1, MUM1, CD10, BCL6, and FOXP1 proteins to subclassify DLBCLs according to the recent Choi algorithm, the authors showed that the genomic profiles observed between the nodal and extranodal DLBCLs were not due to the different proportions of GCB vs ABC in the two groups. Further delineation of these genomic differences was illuminated by the use of high-resolution 21K BAC array CGH performed on 12 independent new cases of extranodal DLBCL. The authors demonstrated for the first time a novel genome and proteome-based signatures that may differentiate the two lymphoma types. |
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Keywords: | diffuse large B-cell lymphoma (DLBCL) nodal DLBCL extranodal DLBCL comparative genomic hybridization (CGH) BCL2 microarray CGH Choi classification ABC GCB GCET1 MUM1 CD10 BCL6 FOXP1 |
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