Methyl tert-butyl ether biodegradation by microbial consortia obtained from soil samples of gasoline-polluted sites in Mexico |
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Authors: | Marcia Morales Elia Velázquez Janet Jan Sergio Revah Uriel González Elías Razo-Flores |
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Affiliation: | Programa de Medio Ambiente y Seguridad, Instituto Mexicano del Petróleo, Eje Central Lazzaro Cárdenas 152, C.P 07730, Mexico DF. mibarria@imp.mx |
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Abstract: | Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when pentane was completely depleted with a cometabolic coefficient of 1 mgMTBE mg(-1)pentane. The analysis of 16S rDNA from isolated microorganisms in the pentane-adapted consortia showed that microorganisms could be assigned to Pseudomonas. This is the first work reporting the cometabolic mineralization of MTBE by consortium of this genus. |
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Keywords: | biodegradation cometabolism consortia methyl tert-butyl ether oxygenated additives Pseudomonas |
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