Interleukin-6 is antiproliferative to a mouse hybridoma cell line and promotive for its antibody productivity |
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Authors: | Fusao Makishima Satoshi Terada Tadashi Mikami Eiji Suzuki |
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Institution: | (1) Institute for Genetics, University of Cologne, Weyertal 121, W-5000 Cologne 41, Germany;(2) Department of Chemical Engineering, Faculty of Engineering, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, 113 Tokyo, Japan |
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Abstract: | Monoclonal antibody production by hybridoma cells at moderately slowed growth states would be favorable for commercial scale production since cells can devote their resources to performing the differentiated function, immunoglobulin production. We found that a purified recombinant human interleukin-6, which had been reported to support or stimulate proliferation of B cell hybridoma/plasmacytoma cells, suppressed growth of a hybridoma cell line in serum-free medium. In the presence of the interleukin, the growth-suppressed cells were viable for remarkably long periods in batch culture, and after removal of the interleukin from the culture medium, they started to proliferate at their normal growth rate. As the concentration of the interleukin increased in the culture, the growth rate decreased and the specific antibody productivity (antibody production rate per cell) increased to 5-fold of control at 10 U ml–1 (2 ng ml–1) of the interleukin.Abbreviations IL-1,2, and 6
interleukin-1, 2 and 6
- rhIL-6
recombinant human interleukin-6
- MCAb
monoclonal antibody
- TNP
trinitrophenyl
- unit (U) of interleukin-6
A unit (U) is equivalent to the amounts of IL-6 which gives one-half maximal IgM secretion by SKW6-CL4 cells (1U ml–1=200 pg ml–1) |
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Keywords: | antibody productivity growth suppression hybridoma interleukin-6 specific productivity |
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