补料发酵法批量培养磁敏感氧化亚铁硫杆菌

磁敏感氧化亚铁硫杆菌胞内可以生成磁性颗粒,探索其培养条件对趋磁细菌的研究有重要意义。利用补料发酵法,实现了磁敏感氧化亚铁硫杆菌的实验室批量培养。发酵终体积为2L的摇瓶发酵条件下,培养40h后一次补Fe2+(5%),使最高菌体浓度达到2.33×107个/ml,比不补料对照提高了60.69%;同样条件下,补入9K全料则可达到2.47×107个/ml,比不补料对照提高70.34%;原子力显微镜磁扫描结果显示发酵得到的菌体有明显磁性,即胞内含有大量磁性颗粒。

Batch Culture of Magnetic Acidthiobacillus ferrooxidans by Fed-batch Fermentation

Acidithiobacillus ferrooxidans (A.f) could synthesize intra-cellular nanometer magnetic particles. With the development of study on their culture conditions, the research of magnetotaxis bacteria will get more benefit from it. By fed-batch fermentation magnetic A.f could be batch cultured in the laboratory. In the shake-flask fermentation, the final volume of liquid was 2 L. After 40 h in the fermentation Fe2+ (5%) was added in the fermentation liquid. The max cell density could reach 2.33×107cells·ml-1. Compared with control experiments, it increased by 60.69%. At the same condition, the completion 9K culture medium was added in the fermentation liquid. The max cell density could reach 2.47×107cells·ml-1. Compared with control experiments, it increased by 70.34%. And atom force microscopy and magnetic force microscopy showed that cells in the fermentation were magnetic.