Effects of acid pre-treatment on bio-hydrogen production and microbial communities during dark fermentation |
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Authors: | Lee Myoung-Joo Song Ji-Hyeon Hwang Sun-Jin |
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Institution: | School of Environment and Applied Chemistry, Center for Environmental Studies, Kyunghee University, Yongin, Republic of Korea. |
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Abstract: | Optimal conditions for acid pre-treatment were investigated for the enrichment of hydrogen-producing bacteria (HPB) in a mixed culture using three strong acids: HCl, HNO(3), and H2SO4 x HCl was selected as a suitable acid for the enrichment of HPB in the fermentation process. The volume of bio-hydrogen produced when the mixed culture was pre-treated using HCl at pH 2 was 3.2 times higher than that obtained without acid pre-treatment. Changes in the microbial community during acid pre-treatment were monitored using images obtained by the fluorescent in situ hybridization (FISH) method and the Live/Dead cell viability test. The tests clearly indicated that the Clostridium species of cluster I were the predominant strains involved in bio-H(2) fermentation, and could be selectively enriched by HCl pre-treatment. |
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