Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by using pulsed-field gel electrophoresis |
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| Authors: | Zhong Wenwan Shou Yulin Yoshida Thomas M Marrone Babetta L |
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| Institution: | Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. |
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| Abstract: | A pulsed-field gel electrophoresis (PFGE) method was developed for discriminating Bacillus anthracis from B. cereus and B. thuringiensis. A worldwide collection of 25 B. anthracis isolates showed high-profile homology, and these isolates were unambiguously distinguished from B. cereus and B. thuringiensis isolates by cluster analysis of the whole-genome macrorestriction enzyme digestion patterns generated by NotI. |
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