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BAC-end Sequence Analysis and a Draft Physical Map of the Common Bean (Phaseolus vulgaris L.) Genome |
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| Authors: | Jessica A Schlueter Jose Luis Goicoechea Kristi Collura Navdeep Gill Jer-Young Lin Yeisoo Yu Dave Kudrna Andrea Zuccolo C Eduardo Vallejos Monica Muñoz-Torres Matthew W Blair Joe Tohme Jeff Tomkins Phillip McClean Rod A Wing Scott A Jackson |
| Institution: | 1. Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA 2. Arizona Genome Institute, Department of Plant Sciences & BIO5, University of Arizona, Tucson, AZ, 86721, USA 3. Department of Horticultural Sciences, University of Florida, Gainesville, FL, 32611, USA 4. International Center for Tropical Agriculture, Cali, Colombia 5. Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29634, USA 6. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA |
| Abstract: | Common bean (Phaseolus vulgaris L.) is a legume that is an important source of dietary protein in developing countries throughout the world. Utilizing the G19833 BAC library for P. vulgaris from Clemson University, 89,017 BAC-end sequences were generated giving 62,588,675 base pairs of genomic sequence covering approximately 9.54% of the genome. Analysis of these sequences in combination with 1,404 shotgun sequences from the cultivar Bat7 revealed that approximately 49.2% of the genome contains repetitive sequence and 29.3% is genic. Compared to other legume BAC-end sequencing projects, it appears that P. vulgaris has higher predicted levels of repetitive sequence, but this may be due to a more intense identification strategy combining both similarity-based matches as well as de novo identification of repeats. In addition, fingerprints for 41,717 BACs were obtained and assembled into a draft physical map consisting of 1,183 clone contigs and 6,385 singletons with ~9x coverage of the genome. |
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