Binding and internalization of immunoglobulin G by cultured human trophoblast |
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Authors: | S R Sooranna S F Contractor |
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Affiliation: | Department of Obstetrics and Gynaecology, Charing Cross and Westminster Medical School, West London Hospital, UK. |
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Abstract: | A preparation consisting of a high percentage of trophoblasts can be obtained by centrifuging a mixture of cell types from trypsinized term placental villi on a discontinuous gradient of Percoll. When cultured these cells maintain trophoblast characteristics as judged by immunocytochemical markers and beta-human chorionic gonadotrophin production. Incubation of cells with labelled human immunoglobulin G demonstrated their ability to bind and internalize this macromolecule in a time- and temperature-dependent manner. |
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